| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Sinorhizobium fredii | AAAGGTCACGGCACTGCT | AAATCGCCTCGTCGCAGA | 59.49 | 59.43 | 152 | 80 |
100.00%
|
100.00%
|
| Primer 42 | Sinorhizobium fredii | ATGCACGTTGTCGGCACA | TCTCGGTGTTGGCGAGTT | 60.59 | 58.87 | 187 | 77 |
100.00%
|
100.00%
|
| Primer 43 | Sinorhizobium fredii | ATGCACGTTGTCGGCACA | GTCTCGGTGTTGGCGAGTT | 60.59 | 60.30 | 188 | 77 |
100.00%
|
100.00%
|
| Primer 44 | Sinorhizobium fredii | TGCGCTTCGGCATCATGA | TCTTGCGTTCGTCGGTGA | 60.13 | 59.28 | 222 | 77 |
100.00%
|
100.00%
|
| Primer 45 | Sinorhizobium fredii | AGTGCGCTTCGGCATCAT | TTCTTGCGTTCGTCGGTGA | 60.12 | 59.93 | 225 | 77 |
100.00%
|
100.00%
|
| Primer 46 | Sinorhizobium fredii | ATCATGCTGGCACGGTTCA | TCATCACGCCAACGAGCA | 60.00 | 59.66 | 235 | 77 |
100.00%
|
100.00%
|
| Primer 47 | Sinorhizobium fredii | ACAGTTGAACGCACGCAG | TGATCGTGGGCAAGAGCA | 58.99 | 58.93 | 197 | 74 |
100.00%
|
100.00%
|
| Primer 48 | Sinorhizobium fredii | GGCATCCTCTTCGCCATGA | AGCAGACGTTCAAGCGGT | 59.85 | 59.58 | 152 | 73 |
100.00%
|
100.00%
|
| Primer 49 | Sinorhizobium fredii | AGGACGCAGACCCAGATCA | TCCCCTCTGTTCCACCGAA | 60.31 | 60.15 | 194 | 73 |
100.00%
|
100.00%
|
| Primer 50 | Sinorhizobium fredii | TCGGTTCGCGTGTTCGAA | GATTTCGTGCGACTCGTGC | 59.97 | 59.94 | 242 | 73 |
100.00%
|
100.00%
|